Histomorphometric analysis of cutaneous remodeling in the early stage of the scleroderma model
AUTOR(ES)
Oliveira, Cristiane Carla de, Velosa, Ana Paula Pereira, Parra, Edwin Roger, Capelozzi, Vera Luiza, Teodoro, Walcy Rosolia, Yoshinari, Natalino Hajime
FONTE
Clinics
DATA DE PUBLICAÇÃO
2009-06
RESUMO
INTRODUCTION/OBJECTIVES: Systemic sclerosis, or scleroderma, is a rheumatic disease characterized by autoimmunity, vasculopathy, and fibrosis of the skin and several internal organs. In the present study, our aim was to assess the skin alterations in animals with scleroderma during the first stages of disease induction. METHODS: To induce scleroderma, female New Zealand rabbits (n = 12) were subcutaneously immunized with 1 mg/ml of collagen V (Col V) in complete Freund's adjuvant, twice with a thirty-day interval. Fifteen days later, the animals received an intramuscular booster with type V collagen in incomplete Freund's adjuvant, twice with a fifteen-day interval. The control group was inoculated with 1 ml of 10 mM acetic acid solution diluted with an equal amount of Freund's adjuvant. Serial dorsal skin biopsies were performed at 7, 15, and 30 days and stained with H&E, Masson's trichrome and Picrosírius for morphological and morphometric analyses. RESULTS: Immunized rabbits presented a significant increase in collagen in skin collected seven days after the first immunization (p=0.05). CONCLUSION: The results from this experimental model may be very important to a better understanding of the pathogenic mechanisms involved in the beginning of human SSc. Therapeutic protocols to avoid early remodeling of the skin may lead to promising treatments for SSc in the future.
Documentos Relacionados
- Histomorphometric assessment of the long-term effects of alendronate on bone quality and remodeling in patients with osteoporosis.
- Genome-Wide Analysis of Survival in Early-Stage Non–Small-Cell Lung Cancer
- The early stages in the degeneration of cutaneous nerve fibres
- Cognition in the Early Stage of Type 2 Diabetes
- Characteristic changes in coronary artery at the early hyperglycaemic stage in a rat type 2 diabetes model and the effects of pravastatin