High-level expression of a chemically synthesized gene for human interferon-gamma using a prokaryotic expression vector.
AUTOR(ES)
Jay, E
RESUMO
A chemically synthesized gene for human interferon-gamma has been cloned into a prokaryotic expression vector under the regulation of a synthetic constitutive transcriptional-translational control unit that contains a strong bacteriophage T5 early promoter and a strong ribosome-binding site. Cells harboring the recombinant plasmid express high levels (4 X 10(9) units per liter of culture) of antiviral activity specific for interferon-gamma. Analysis of total cell lysates on NaDodSO4/polyacrylamide gels revealed a 17,200-dalton protein, expected for the nonglycosylated form of human interferon-gamma, that constitutes greater than 15% of total cell protein.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=345044Documentos Relacionados
- High-level production of recombinant proteins in CHO cells using a dicistronic DHFR intron expression vector.
- High-level expression of a cloned HLA heavy chain gene introduced into mouse cells on a bovine papillomavirus vector.
- Ping-pong amplification of a retroviral vector achieves high-level gene expression: human growth hormone production.
- Rapid, high-level expression of biologically active alpha-trichosanthin in transfected plants by an RNA viral vector.
- A human beta-actin expression vector system directs high-level accumulation of antisense transcripts.