High-efficiency transformation of Streptococcus lactis protoplasts by plasmid DNA.

AUTOR(ES)

Simon, D

RESUMO

Streptococcus lactis IL1403 protoplasts were transformed by plasmid pIL204 (5.5 kilobases), which conferred erythromycin resistance with an average efficiency of 5 X 10(6) transformants per microgram of supercoiled DNA. The procedure used and transformation efficiencies obtained were close to those described for Bacillus subtilis (G. Chang and S. N. Cohen, Mol. Gen. Genet. 168:111-115, 1979).

Documentos Relacionados

• High-efficiency transformation of mammalian cells by plasmid DNA.
• Short-term, high-efficiency expression of transfected DNA.
• High-efficiency transformation of bacterial cells by electroporation.
• High-Efficiency Transformation of Rhizobium leguminosarum by Electroporation
• Characterization of genetic transformation in Streptococcus mutans by using a novel high-efficiency plasmid marker rescue system.