High CO2 Requiring Mutant of Anacystis nidulans R21

AUTOR(ES)

Marcus, Yehouda

RESUMO

Some physiological characteristics of a mutant (E1) of Anacystis nidulans R2, incapable of growing at air level of CO2, are described. E1 is capable of accumulating inorganic carbon (Ci) internally as efficiently as the wild type (R2). The apparent photosynthetic affinity for Ci in E1, however, is some 1000 times lower than that of R2. The kinetic parameters of ribulose 1,5-bisphosphate carboxylase/oxygenase from E1 are similar to those observed in R2. The mutant appears to be defective in its ability to utilize the intracellular Ci pool for photosynthesis and depends on extracellular supply of Ci in the form of CO2. The very high apparent photosynthetic Km (CO2) of the mutant indicate a large diffusion resistance for CO2. Data obtained here are used to calculate the permeability coefficient for CO2 between the bulk medium and the carboxylation site of cyanobacteria.

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