Heterologous Expression of a Putative ClpC Chaperone Gene Leads to Induction of a Host Metabolite
AUTOR(ES)
Braesel, Jana
FONTE
J. Braz. Chem. Soc.
DATA DE PUBLICAÇÃO
2019-03
RESUMO
Genome mining provides exciting opportunities for the discovery of natural products. However, in contrast to traditional bioassay-guided approaches, challenges of genome mining include poor or no expression of biosynthetic gene clusters (BGCs). Additionally, given that thousands of BGCs are now available through extensive genome sequencing, how does one select BGCs for discovery? Synthetic biology techniques can be used for BGC refactoring and activation, whereas resistance-gene-directed genome mining is a promising approach to discover bioactive natural products. Here we report the selection of a BGC by applying a resistance-gene-directed approach, cloning of the silent BGC from Micromonospora sp. B006, promoter exchange, and heterologous expression in Streptomyces coelicolor M1152. While we have yet to identify the encoded compound, we unexpectedly observed induction of a host metabolite, which we hypothesize is due to the presence of a caseinolytic protease C (ClpC) chaperone gene in the BGC, suggesting that ClpC chaperones may be used for BGC activation.
Documentos Relacionados
- MecA, an adaptor protein necessary for ClpC chaperone activity
- Stress induction of clpC in Bacillus subtilis and its involvement in stress tolerance.
- Regulation and Physiological Significance of ClpC and ClpP in Streptococcus mutans
- MecB of Bacillus subtilis, a member of the ClpC ATPase family, is a pleiotropic regulator controlling competence gene expression and growth at high temperature.
- ClpC ATPase Is Required for Cell Adhesion and Invasion of Listeria monocytogenes