Hepatitis C virus genotypes in blood donors from the Federal District, Central Brazil
Amorim, Regina MS, Oliveira, Claudiner P, Wyant, Patrícia S, Cerqueira, Daniela M, Câmara, Geni NL, Flores, Luciano S, Martins, Regina MB, Martins, Cláudia RF
Memórias do Instituto Oswaldo Cruz
DATA DE PUBLICAÇÃO
The objective of this study was to characterize hepatitis C virus (HCV) genotypes in blood donors from the Federal District, Central Brazil, and to compare HCV screening by serological assays and reverse transcriptase polymerase chain reaction (RT-PCR). Plasma samples from 57 individuals with reactive or indeterminate results in serological anti-HCV screening assays (ELISA or EIA) were tested for HCV RNA by RT-PCR. The results from a confirmatory LIA serological assay were also evaluated. The 5' non-coding region of the HCV genome was amplified from 41 PCR positive samples (71.9%), which were further characterized by nucleotide sequencing analysis. Of these, 60.9% were of HCV genotype 1 and 39.1% of genotype 3.
- Prevalence and genotypes of GB Virus C/Hepatitis G virus among blood donors in Central Brazil
- Antiretroviral resistance and genetic diversity of human immunodeficiency virus type 1 isolates from the Federal District, Central Brazil
- Prevalence of human papillomavirus type 16 variants in the Federal District, Central Brazil
- Hepatitis C virus in blood samples from volunteer donors.
- Geographical distribution of hepatitis C virus genotypes in blood donors: an international collaborative survey.