Heparin inhibits EcoRI endonuclease cleavage of DNA at certain EcoRI sites.
AUTOR(ES)
Chen, J Z
RESUMO
Studies presented here demonstrate that heparin inhibits EcoRI endonuclease cleavage of DNA whereas related proteoglycans show no effect. The inhibition occurs at particular EcoRI sites that are near or overlap with palindromic sequences in the murine lambda 5 and Lyt-2 genes. Endogenous heparin from peritoneal mast cells co-isolates with DNA and inhibits digestion of peritoneal cell DNA at the inhibitable sites. Digestion of spleen DNA is inhibited at the same sites when commercial heparin is added prior to digestion. In both cases, the inhibition is abolished by pre-treating the DNA with heparinase. Thus, potential artifacts in restriction fragment length analyses could occur with DNA isolated either from cells that are naturally rich in heparin or from cells to which heparin has been added, e.g., as an anticoagulant.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=330931Documentos Relacionados
- Structural adaptations in the interaction of EcoRI endonuclease with methylated GAATTC sites.
- Cleavage of Replicating Forms of Mitochondrial DNA by EcoRI Endonuclease
- Cleavage of single stranded oligonucleotides by EcoRI restriction endonuclease.
- Determinants of EcoRI endonuclease sequence discrimination.
- Facilitated distortion of the DNA site enhances EcoRI endonuclease-DNA recognition.