HemoTypeSC point-of-care testing shows high sensitivity with alkaline cellulose acetate hemoglobin electrophoresis for screening hemoglobin SS and SC genotypes
AUTOR(ES)
Adegoke, Samuel Ademola; Oladimeji, Oluwatoyin Ibukun; Akinlosotu, Morenike Agnes; Akinwumi, Alex Ifeoluwa; Matthew, Kayode Ademola
FONTE
Hematology, Transfusion and Cell Therapy
DATA DE PUBLICAÇÃO
2022
RESUMO
ABSTRACT Introduction: By providing timely actionable results for prompt management, point-of-care testing (POCT) kits have revolutionised medical care for various diseases, ranging from infectious diseases like malaria to genetic disorders, such as sickle cell disease (SCD). They are, however, underutilised in the diagnosis of SCD in developing countries, where the need is greatest. Objective: The study was aimed at assessing the sensitivity of HemoTypeSC POCT among a cohort of children with SCD, previously diagnosed by Alkaline cellulose acetate hemoglobin electrophoresis (ACAE), with or without high-performance liquid chromatography (HPLC). Methods: In this descriptive cross-sectional study, HemoTypeSC test was conducted on all participants and its sensitivity was determined by comparing results with those obtained using ACAE. Discordance was verified with HPLC. Results: One hundred and forty-five children aged one to 19 years were studied. There were 84 males and 61 females (male: female ratio = 1.4:1). The HemoTypeSC was able to correctly diagnose sickle cell anemia (SCA) and hemoglobin SC in all (100%) of the children tested. Conclusion: The HemoTypeSC shows high sensitivity in detecting SCA and hemoglobin SC. Hence, it is useful for targeted screening of individuals suspected of having SCD, leading to rapid diagnosis of these hemoglobinopathies, even in resource-constrained settings.
Documentos Relacionados
- Point-of-Care Testing of Hemostasis in Cardiac Surgery
- Hemoglobin A1c Point-of-Care Assays; a New World with a Lot of Consequences!
- Smartphone for Point-of-Care Quantification of Protein by Bradford Assay
- Usefulness of Point-of-Care Testing in the Treatment of Diabetes in an Underserved Population
- RNI Point-of-care test (POCT): esperança ou ilusão?