HEMA/MMA/EDMA packing material evaluation for size exclusion chromatography (SEC)

AUTOR(ES)

Vianna-Soares, Cristina Duarte, Cherng-Ju, Kim, Borenstein, Michael Robert

FONTE

Materials Research

DATA DE PUBLICAÇÃO

2005-03

RESUMO

HEMA/MMA/EDMA (2-hydroxyethyl methacrylate/methyl methacrylate/ethyleneglycol methacrylate) copolymer beads have been synthesized for use in aqueous SEC. This porous chromatographic support consisted of a large average particle size (250 µm) and contained macropores with a median pore size of approximately 880 Å. The material was packed in glass and stainless columns to assess their chromatographic performance. The packed columns were calibrated using narrow molecular weight (MW) distribution standards (dextran) and exhibited a range of molecular weight separation between 40,000 and 2,000,000 daltons. The packing material showed the ability to separate large molecules through the size exclusion mechanism.

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