Helper-independent retrovirus vectors with Rous-associated virus type O long terminal repeats.

AUTOR(ES)

Greenhouse, J J

RESUMO

We have constructed nonpermuted replication-competent avian retrovirus vectors that derive from Rous sarcoma virus (S. H. Hughes, J. J. Greenhouse, C. J. Petropoulos, and P. Sutrave, J. Virol. 61:3004-3012, 1987). We describe here the construction and properties of corresponding vectors in which the long terminal repeats (LTRs) of the parental virus have been replaced by the LTRs of the endogenous chicken virus Rous-associated virus type O. The Rous-associated virus type O LTR vectors replicated approximately 1/10 as well as the parental vectors and expressed a test gene, chloramphenicol acetyltransferase, approximately 1/30 to 1/50 as well.

Documentos Relacionados

• Sequences outside of the long terminal repeat determine the lymphomogenic potential of Rous-associated virus type 1.
• Helper-independent transformation by unintegrated Harvey sarcoma virus DNA.
• Integration of Rous-associated virus type O provirus in susceptible chicken cells.
• Adaptor plasmids simplify the insertion of foreign DNA into helper-independent retroviral vectors.
• Sequence of the Long Terminal Repeat and Adjacent Segments of the Endogenous Avian Virus Rous-Associated Virus 0