Heat inactivation of catalase from Staphylococcus aureus MF-31.
AUTOR(ES)
Andrews, G P
RESUMO
The effects of heat on catalase from Staphylococcus aureus lysates were examined. Catalase activity increased with increasing concentrations of potassium phosphate buffer, when heated at temperatures between 50 and 65 degrees C for 10 min. Inactivation of catalase by NaCl during heating was demonstrated. Extended heating of S. aureus cells at 52 degrees C resulted in a slight decrease in catalase activity of the resultant lysates. This decrease was more pronounced in the presence of salt. Heating at 62 degrees C caused a decrease in catalase activity, but not complete inactivation. These results implicate the combined effects of heat, and NaCl in the inactivation of catalase from S. aureus. The findings are consistent with the hypothesis that H2O2 may accumulate as a result of decreased catalase activity and be responsible for the decreased colony-forming ability of stressed S. aureus.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=243375Documentos Relacionados
- Synthesis of catalase in Staphylococcus aureus MF-31.
- Catalase activity during the recovery of heat-stressed Staphylococcus aureus MF-31.
- Superoxide dismutase activity during recovery of thermally stressed Staphylococcus aureus MF-31.
- Effect of free-radical scavengers on enumeration of thermally stressed cells of Staphylococcus aureus MF-31.
- Coagulase Production by Injured Staphylococcus aureus MF-31 During Recovery1
