Haplotypes of vitamin D modulate the circulate levels of leaf in exposed subjects / Modulação toxicogenetica das concentrações sanguineas e plasmaticas de chumbo por haplotipos do receptor da vitamina D

AUTOR(ES)
DATA DE PUBLICAÇÃO

2009

RESUMO

The Vitamin D Receptor (VDR) plays an important role in the toxicity of lead (Pb). Genetic factors, i.e polymorphisms, influence blood lead (Pb-B) concentrations in lead exposed subjects. However, only a few research studies have evaluated the effect of VDR polymorphism on the lead concentration in environmentally. Also, no studies have evaluated the combinatorial effect of these polymorphisms on the same parameters. The haplotype analysis (combination of genetic indicators in a certain region of the chromossome) has been shown to be a better tool if compared with the analyses of single polymorphisms. This study aimed at examining the combined effects (haplotype analysis) of three polymorphisms (BsmI , ApaI and FokI) in vitamin D receptor ( VDR) gene on Pb-B and on the concentrations of lead in plasma (Pb-P), which is more relevant to lead toxicity, in 150 environmentally exposed subjects (65 mens and 85 women). Genotypes were determined by RFLP, and Pb-P and Pb-B were determined by inductively coupled plasma mass spectrometry and by graphite furnace atomic absorption spectrometry, respectively. Subjects with the bb (BsmI ) or ff (FokI) genotypes have lower B-Pb than subjects in the other genotype groups. Subjects with the aa (ApaI ) or ff genotypes have lower P-Pb than subjects in the other genotype groups. Lower Pb-P, Pb-B, and %Pb-P/Pb-B levels were found in subjects with the haplotype combining the a, b, and f alleles for the ApaI , BsmI , and FokI polymorphisms, respectively, compared with the other haplotype groups. These findings may have important toxicogenetic implications and their molecular basis needs to be addressed in further studies

ASSUNTO(S)

polimorfismo (genetica) polymorphism (genetica) lead vitamin d chumbo vitamina d

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