gyrA Polymorphism in Campylobacter jejuni: Detection of gyrA Mutations in 162 C. jejuni Isolates by Single-Strand Conformation Polymorphism and DNA Sequencing
AUTOR(ES)
Hakanen, Antti
FONTE
American Society for Microbiology
RESUMO
Mutations in the quinolone resistance-determining region of the gyrA gene from 138 ciprofloxacin-resistant (MIC, ≥4 μg/ml) and 24 ciprofloxacin-susceptible (MIC, ≤1 μg/ml) clinical Campylobacter jejuni isolates were subjected to single-strand conformation polymorphism analysis and sequencing. All of the isolates could be assigned to three genotypic clusters based on silent mutations. All resistant isolates had a point mutation at codon 86.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=127378Documentos Relacionados
- Detection of novel mutations in the gyrA gene of Staphylococcus aureus by nonradioisotopic single-strand conformation polymorphism analysis and direct DNA sequencing.
- Detection of gyrA and gyrB mutations in quinolone-resistant clinical isolates of Escherichia coli by single-strand conformational polymorphism analysis and determination of levels of resistance conferred by two different single gyrA mutations.
- Detection of Rifampin-Resistant Mycobacterium tuberculosis in Sputa by Nested PCR-Linked Single-Strand Conformation Polymorphism and DNA Sequencing
- Screening for mutations by RNA single-strand conformation polymorphism (rSSCP): comparison with DNA-SSCP.
- Two dimensional single-strand conformation polymorphism analysis: a useful tool for the detection of mutations in long DNA fragments.