Growth of and omega-3 fatty acid production by Phaeodactylum tricornutum under different culture conditions.
Detailed studies were carried out on the effects of nitrogen source, phosphate, sodium chloride, growth factors, precursors, CO2, temperature, initial pH, and inoculum size on biomass and eicosapentaenoic acid (EPA) production by Phaeodactylum tricornutum. The EPA content of total fatty acids increased with increasing concentrations of nitrate and urea. Sodium chloride was not required for growth or EPA production. While vitamins B1 and B12 did not affect growth significantly, EPA yield was increased by 65% by B12 supplementation. Maximum EPA production occurred when the air gassing supply was supplemented with 1% CO2. Optimum culture temperature and initial pH for EPA production were 21.5 to 23 degrees C and 7.6, respectively. EPA yields of up to 133 mg/liter of culture were observed. EPA constituted up to 30 to 40% of total fatty acids.
ACESSO AO ARTIGOhttp://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=182726
- Omega-3 fatty acid supplementation in horses
- Cloning of higher plant omega-3 fatty acid desaturases.
- Plasmid omega-3 fatty acid desaturase cDNA from Ricinus communis.
- Dietary omega-3 fatty acid deficiency and visual loss in infant rhesus monkeys.
- Dietary Omega-3 Fatty Acid Deficiency and Visual Loss in Infant Rhesus Monkeys