Growth, cysts and kinetics of Borrelia garinii (Spirochaetales: Spirochaetacea) in different culture media

AUTOR(ES)

Oliveira, Angela de, Fonseca, Adivaldo Henrique, Costa, Catia Marques da, Mantovani, Elenice, Yoshinari, Natalino Hajime

FONTE

Memórias do Instituto Oswaldo Cruz

DATA DE PUBLICAÇÃO

2010-08

RESUMO

The aim of the present paper was to evaluate cyst formation and growth parameters of Borrelia garinii in a range of media differing in formulation and cost. A qualitative assessment of morphology and motility of B. garinii was conducted. All media were prepared aseptically and used in test tubes or Petri dishes. For each medium, the initial spirochete concentration was standardized to 10³ spirochets/mL. The following culture media were suitable to grow B. garinii: Barbour-Stoenner-Kelly, brain heart infusion and PMR. Growth was minimal at six weeks post-inoculation and maximum spirochete density was observed between 9-12 weeks. Often, the cultures developed cysts of different sizes, isolated or in groups, with a spiraled portion of variable sizes, mainly in unfavorable culture media. Brazilian Lyme disease-like illness, also known as Baggio-Yoshinari syndrome (BYS), is a new and interesting emerging tick-borne disease, caused by Borrelia burgdorferi sensu lato spirochetes, only during its cystic forms. It has been assumed that the peculiar clinical and laboratory features of BYS are consequential to the absence of a human sucker Ixodes ricinus complex tick at risk areas in Brazil, supporting the concept that the borrelia phenotypic expression pattern is modified as it is transmitted through the host.

Documentos Relacionados

• In Vitro Culture of Borrelia garinii Results in Loss of Flagella and Decreased Invasiveness
• Culture media profoundly affect Candida albicans and Candida tropicalis growth, adhesion and biofilm development
• Mycelial growth and sporulation of Apoharknessia eucalyptorum on different culture media
• Kinetics of phosphate uptake, growth, and accumulation of cyclic diphosphoglycerate in a phosphate-limited continuous culture of Methanobacterium thermoautotrophicum.
• Association of Borrelia garinii and B. valaisiana with Songbirds in Slovakia