Groucho acts as a corepressor for a subset of negative regulators, including Hairy and Engrailed

AUTOR(ES)

Jiménez, Gerardo

FONTE

Cold Spring Harbor Laboratory Press

RESUMO

Relatively little is known about the molecular mechanisms involved in transcriptional repression, despite its importance in development and differentiation. Recent evidence suggests that some transcriptional repressors act by way of adaptor molecules known as corepressors. Here, we use in vivo functional assays to test whether different repressor activities are mediated by the Groucho (Gro) corepressor in the Drosophila embryo. Previously, Gro was proposed to mediate repression by the Hairy-related family of basic helix–loop–helix proteins. Our results indicate not only that repression by Hairy requires Gro, but that a repressor domain from the Engrailed (En) homeodomain protein is also Gro dependent. The latter result correlates with an ability of this En domain to bind to Gro in vitro. In contrast, repressor regions from the Even-skipped, Snail, Krüppel, and Knirps transcription factors are effective in the absence of Gro. These results show that Gro is not generally required for repression, but acts as a specific corepressor for a fraction of negative regulators, including Hairy and En.

Documentos Relacionados

• Two Distinct Types of Repression Domain in Engrailed: One Interacts with the Groucho Corepressor and Is Preferentially Active on Integrated Target Genes
• Regulators, culture media and types of lights in vitro lavender culture
• Dmrt1, a gene related to worm and fly sexual regulators, is required for mammalian testis differentiation
• A human peripheral blood monocyte-derived subset acts as pluripotent stem cells
• Conversion of Dorsal from an activator to a repressor by the global corepressor Groucho