Glycolic Acid Labeling During Photosynthesis with 14CO2 and Tritiated Water

AUTOR(ES)

Plamondon, J. E.

RESUMO

Chlorella pyrenoidosa were allowed to photosynthesize for short periods of time in the presence of 14CO2 and HTO. Analysis of tritium and 14C labeling of photosynthetic intermediate compounds showed that the T/14C ratio of glycolic acid was comparable to that of intermediate compounds of the photosynthetic carbon reduction cycle when photosynthesis was performed in nearly 100% oxygen and only slightly higher under steady-state conditions. It is concluded that formation of labeled glycolic acid as a consequence of its proposed hydrogen transport role in photosynthesis is quantitatively of limited importance compared to the net synthesis of glycolic acid from CO2.

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