Germ line transmission of an inactive N-myc allele generated by homologous recombination in mouse embryonic stem cells.

AUTOR(ES)

Stanton, B R

RESUMO

We have disrupted one allele of the N-myc locus in mouse embryonic stem (ES) cells by using homologous recombination techniques and have obtained germ line transmission of null N-myc ES cell lines with transmission of the null N-myc allele to the offspring. The creation of mice with a deficient N-myc allele will allow the generation of offspring bearing null N-myc alleles in both chromosomes and permit study of the role that this proto-oncogene plays in embryonic development.

Documentos Relacionados

• High-frequency disruption of the N-myc gene in embryonic stem and pre-B cell lines by homologous recombination.
• Germ-line transmission of a planned alteration made in a hypoxanthine phosphoribosyltransferase gene by homologous recombination in embryonic stem cells.
• Characterization of N-myc amplification units in human neuroblastoma cells.
• Excision of N-myc from chromosome 2 in human neuroblastoma cells containing amplified N-myc sequences.
• Rapid and transient decrease of N-myc expression in retinoic acid-induced differentiation of OTF9 teratocarcinoma stem cells.