Genotypic Diversity among Strains of Bradyrhizobium japonicum Belonging to Serogroup 110
AUTOR(ES)
van Berkum, P.
RESUMO
Thirty-three strains of Bradyrhizobium japonicum within serogroup 110 were examined for genotypic diversity by using DNA-DNA hybridization analyses. The analysis of the DNA from 15 hydrogen-uptake-negative strains with the bradyrhizobial uptake hydrogenase probe pHU52 showed variation in degree of homology and restriction fragment length polymorphism of EcoRI-restricted DNA. Clustering analysis of the 33 strains on the basis of DNA-DNA hybridization analysis with four restriction enzymes and with the bradyrhizobial nodulation locus, pRJUT10, as probe indicated the existence of four groups of strains, which were less than 70% similar. Restriction digestion of genomic DNA with BamHI and DNA-DNA hybridization with pRJUT10 permitted classification of each of the strains according to a specific fingerprint pattern.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=182418Documentos Relacionados
- Phenotypic Diversity among Strains of Bradyrhizobium japonicum Belonging to Serogroup 110
- Genetic Diversity in Bradyrhizobium japonicum Serogroup 123 and Its Relation to Genotype-Specific Nodulation of Soybean
- Genotypic Diversity among Brevibacillus laterosporus Strains
- Bradyrhizobium japonicum Serocluster 123 and Diversity among Member Isolates †
- Serological and genotypic diversity among serogroup 5- reacting environmental Legionella isolates.
