Genotypic and phenotypic characterization of nonadherent mutants of atypical enteropathogenic Escherichia coli of serotype 0125ac:H6. / Caracterização genotípica e fenotípica de mutantes não aderentes de Escherichia coli enteropatogênica atípica do sorotipo O125ac:H6.

AUTOR(ES)
DATA DE PUBLICAÇÃO

2009

RESUMO

The serotype O125ac:H6 comprises atypical Enteropathogenic Escherichia coli strains that express the aggregative adherence (AA) pattern to HEp-2 cells. We obtained four nonadherent mutants using TnphoA insertion in the Ec292/84 strain. The aim of this study was the genetic and phenotypic characterization of these mutants. The genetic analysis of the mutants revealed that the insertion of TnphoA ocurred in the secD gene, part of the bacterial type 2 secretion system (T2SS). The mutant outer membrane proteins (OMP) profile, in comparison to the prototype strain, demonstrated the lack of expression of proteins of 21 and 30 kDa in the mutant profile. An antiserum raised against the OMP extract of the prototype strain, in addition to inhibit the AA pattern, recognized the 30 kDa protein in immunoblotting assays with OMP extract. These data indicate this OMP is involved in the establishment of the AA pattern presented by the atypical EPEC strains of the O125ac:H6 serotype, and that this protein is transported via the T2SS.

ASSUNTO(S)

escherichia coli diarrhea membrane protein proteínas de membrana escherichia coli diarréia sistemas de secreção secretion system

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