Genotoxicity of Nicotiana tabacum leaves on Helix aspersa
AUTOR(ES)
Silva, Fernanda R. da, Erdtmann, Bernardo, Dalpiaz, Tiago, Nunes, Emilene, Ferraz, Alexandre, Martins, Tales L.C., Dias, Johny F., Rosa, Darlan P. da, Porawskie, Marilene, Bona, Silvia, Silva, Juliana da
FONTE
Genet. Mol. Biol.
DATA DE PUBLICAÇÃO
17/05/2013
RESUMO
Tobacco farmers are routinely exposed to complex mixtures of inorganic and organic chemicals present in tobacco leaves. In this study, we examined the genotoxicity of tobacco leaves in the snail Helix aspersa as a measure of the risk to human health. DNA damage was evaluated using the micronucleus test and the Comet assay and the concentration of cytochrome P450 enzymes was estimated. Two groups of snails were studied: one fed on tobacco leaves and one fed on lettuce (Lactuca sativa L) leaves (control group). All of the snails received leaves (tobacco and lettuce leaves were the only food provided) and water ad libitum. Hemolymph cells were collected after 0, 24, 48 and 72 h. The Comet assay and micronucleus test showed that exposure to tobacco leaves for different periods of time caused significant DNA damage. Inhibition of cytochrome P450 enzymes occurred only in the tobacco group. Chemical analysis indicated the presence of the alkaloid nicotine, coumarins, saponins, flavonoids and various metals. These results show that tobacco leaves are genotoxic in H. aspersa and inhibit cytochrome P450 activity, probably through the action of the complex chemical mixture present in the plant.
Documentos Relacionados
- Developmental Instability in Leaves and Flowers of NICOTIANA TABACUM
- Comprehensive analysis of gene expression in Nicotiana tabacum leaves acclimated to oxidative stress
- Induction of UDP-Glucose:Salicylic Acid Glucosyltransferase Activity in Tobacco Mosaic Virus-Inoculated Tobacco (Nicotiana tabacum) Leaves.
- Calcium current inactivation in identified neurones of Helix aspersa.
- A cholinergic chloride conductance in neurones of Helix aspersa.
