Genotoxic evaluation of an industrial effluent from an oil refinery using plant and animal bioassays
AUTOR(ES)
Rodrigues, Fernando Postalli, Angeli, José Pedro Friedmann, Mantovani, Mário Sérgio, Guedes, Carmen Luisa Barbosa, Jordão, Berenice Quinzani
FONTE
Genetics and Molecular Biology
DATA DE PUBLICAÇÃO
29/01/2010
RESUMO
Polycyclic aromatic hydrocarbons (PAHs) are genotoxic chemicals commonly found in effluents from oil refineries. Bioassays using plants and cells cultures can be employed for assessing environmental safety and potential genotoxicity. In this study, the genotoxic potential of an oil refinery effluent was analyzed by means of micronucleus (MN) testing of Alium cepa, which revealed no effect after 24 h of treatment. On the other hand, primary lesions in the DNA of rat (Rattus norvegicus) hepatoma cells (HTC) were observed through comet assaying after only 2 h of exposure. On considering the capacity to detect DNA damage of a different nature and of these cells to metabolize xenobiotics, we suggest the association of the two bioassays with these cell types, plant (Allium cepa) and mammal (HTC) cells, for more accurately assessing genotoxicity in environmental samples.
Documentos Relacionados
- Genotoxic and mutagenic effects of polluted surface water in the midwestern region of Brazil using animal and plant bioassays
- Comparison between four and seven-day Ceriodaphnia dubia survival and reproduction test protocols using oil refinery effluent samples
- Genotoxicity assessment of a pharmaceutical effluent using four bioassays
- Adsorption of residual oil from palm oil mill effluent using rubber powder
- Biodegradation of oil refinery residues using mixed-culture of microorganisms isolated from a landfarming