Genomic cloning and characterization of a ricin gene from Ricinus communis.
AUTOR(ES)
Halling, K C
RESUMO
A genomic clone that specifies a single polypeptide precursor for ricin, a toxic lectin of Ricinus communis (castor bean), was isolated, sequenced and Sl mapped. The gene encodes a 64 kDa precursor which contains, in the following order: a 24 or 35 amino acid signal peptide, the A chain, a 12 amino acid linker peptide, and the B chain. The 5'-end of the ricin mRNA maps approximately 35 bases upstream from the first methionine codon. Two putative TATA boxes and a possible CAAT box lie in the 5'-flanking region. Two possible polyadenylation signals were found in the 3' flanking region. No introns were found, which is typical of other lectin genes that have been sequenced. Southern blot analysis suggests that the castor bean genome contains approximately six ricin-like genes.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=322107Documentos Relacionados
- Isolation of a full-length cDNA encoding cytosolic enolase from Ricinus communis.
- Plasmid omega-3 fatty acid desaturase cDNA from Ricinus communis.
- Fat metabolism in higher plants. XXIV. A soluble beta-oxidative system from germinating seeds of Ricinus communis.
- Fat metabolism in higher plants. XXV. The enzymic degradation of hydroxy long chain fatty acids by extracts of Ricinus communis.
- Desenvolvimento de biossensores eletroquímicos baseados em filmes de xiloglucana para detecção de Ricina e Ricinus communis aglutinina.