Genome-wide identification and characterization of R2R3-MYB genes in Medicago truncatula
AUTOR(ES)
Li, Wei
FONTE
Genet. Mol. Biol.
DATA DE PUBLICAÇÃO
14/11/2019
RESUMO
Abstract MYB is a large family of plant transcription factors. Its function has been identified in several plants, while there are few reports in Medicago truncatula. In this study, we used RNA-seq data to analyze and identify R2R3-MYB genes in the genome of Medicago truncatula. Phylogenetic analysis classified 150 MtMYB genes into 21 subfamilies with homologs. Out of the 150 MtMYB genes, 139 were distributed among 8 chromosomes, with tandem duplications (TD) and segment duplications (SD). Microarray data were used for functional analysis of the MtMYB genes during growth and developmental processes providing evidence for a role in tissues differentiation, seed development processes, and especially the nodulation process. Furthermore, we investigated the expression of MtMYB genes in response to abiotic stresses using RNA-seq data, which confirmed the critical roles in signal transduction and regulation processes under abiotic stress. We used quantitative real-time PCR (qRT-PCR) to validate expression profiles. The expression pattern of M. truncatula MYB genes under different abiotic stress conditions suggest that some may play a major role in cross-talk among different signal transduction pathways in response to abiotic stresses. Our study will serve as a foundation for future research into the molecular function of M. truncatula R2R3-MYB genes.
Documentos Relacionados
- Genome-Wide Identification of Nodule-Specific Transcripts in the Model Legume Medicago truncatula1
- Genome-Wide Medicago truncatula Small RNA Analysis Revealed Novel MicroRNAs and Isoforms Differentially Regulated in Roots and Nodules[W]
- Genome-Wide Identification of Potential Plant E2F Target Genes1[w]
- Genome-wide identification of genes likely to be involved in human genetic disease
- Identification of Novel Imprinted Genes in a Genome-Wide Screen for Maternal Methylation
