Genic expression of uterine leiomyoma in women during the reproductive age after treatment with agonist GnRH analogue / Expressão gênica do leiomioma uterino em mulheres no período reprodutivo após tratamento com análogo agonista do GnRH

AUTOR(ES)

Rodrigo Borsari

DATA DE PUBLICAÇÃO

2008

RESUMO

OBJECTIVE: To identify the genes differentially expressed between the uterine leiomyomas of patients in reproductive age, treat or not treated with GnRH analogue and confirm the results for genes selected by real time PCR. METHODS: It was harvested sample of the largest lump of uterine leiomyoma of 89 black patients, aged between 20 and 45 years, with details of surgical miomectomia. Of these, 38 patients received GnRH analogue prior to surgery (Group A) and 51 were undergoing surgery without prior treatment (Group B). Within each group were selected 10 patients nulliparous, with higher nodule over 3.0 cm, uterine volume over 300 cc. and samples taken during lutea in Group B. INTERVENTION: 5 samples from each group were analyzed by microarray, and then differentially expressed genes were analyzed by real time PCR on 10 patients in each group. RESULTS: Of the 47,000 sequences of the platform used, representing around 38,500 human genes already characterized, resulted in differential expression of 174 genes, with 70 genes up regulated (33 genes with known function) and 104 down regulated (65 genes with known function) in samples of Group A (Treaty) compared to Group B (Non-Treaty). The up regulated genes CYR 61, EGR 1 and SULF 2 and down regulated, WIF 1 were confirmed by real time PCR , while the gene HMGN1 had no confirmation of expression after real-time PCR. CONCLUSIONS: There is change in the gene expression of uterine leiomyoma of women undergoing treatment with GnRH analogue regarding women not treated. The number of genes underexpressed genes is double the down regulated and 80% of the changes detected by microarray were confirmed by real time PCR.

ASSUNTO(S)

expressão gênica polymerase chain reaction reação em cadeia de polimerase leiomioma gonadotropin release hormone hormônio liberador de gonadotropina microarray genic expression análise em microsséries leiomyoma

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