Genetic variability in the 5' UTR and NS5A regions of hepatitis C virus RNA isolated from non-responding and responding patients with chronic HCV genotype 1 infection

AUTOR(ES)

Araújo, Flávio MG, Sonoda, Ivan V, Rodrigues, Nilton B, Teixeira, Rosangela, Redondo, Rodrigo AF, Oliveira, Guilherme C

FONTE

Memórias do Instituto Oswaldo Cruz

DATA DE PUBLICAÇÃO

2008-09

RESUMO

Sequence variation among different hepatitis C virus (HCV) isolates has adaptive significance and reflects the modes and intensities of selection mechanisms operating on the virus. In this work, we sought to investigate using classical population genetics parameters, the genetic variability of HCV genotype 1 using the 5' UTR and NS5A regions from treatment non-responding and responding groups of patients. Both regions showed low genetic varia-bility and the 5' UTR showed neutral deviation. No differences were observed in the nonsynonymous/synonymous nucleotide substitution ratio among groups for NS5A. The analysis of molecular variance test of the 5' UTR region showed an 11.94% variation among groups. Phylogenetic analysis showed no correlation between sequence variations and therapeutic responses.

Documentos Relacionados

• Sequencing of E2 and NS5A regions of HCV genotype 3a in Brazilian patients with chronic hepatitis
• Effect of Interaction between Hepatitis C Virus NS5A and NS5B on Hepatitis C Virus RNA Replication with the Hepatitis C Virus Replicon
• Amphipathic Helix-Dependent Localization of NS5A Mediates Hepatitis C Virus RNA Replication
• Antigenic structure of the complete nonstructural (NS) 2 and 5 proteins of hepatitis C virus (HCV): anti-HCV NS2 and NS5 antibody reactivities in relation to HCV serotype, presence of HCV RNA, and acute HCV infection.
• "Sequenciamento da região NS5A do genoma do vírus da hepatite C, genótipo 3, de pacientes brasileiros com infecção crônica"