Genetic transformation of the plant pathogens Phytophthora capsici and Phytophthora parasitica.
AUTOR(ES)
Bailey, A M
RESUMO
Phytophthora capsici and P.parasitica were transformed to hygromycin B resistance using plasmids pCM54 and pHL1, which contain the bacterial hygromycin B phosphotransferase gene (hph) fused to promoter elements of the Ustilago maydis heat shock hsp70 gene. Enzymes Driselase and Novozyme 234 were used to generate protoplasts which were then transformed following exposure to plasmid DNA and polyethylene glycol 6000. Transformation frequencies of over 500 transformants per micrograms of DNA per 1 x 10(6) protoplasts were obtained. Plasmid pCM54 appears to be transmitted in Phytophthora spp. as an extra-chromosomal element through replication, as shown by Southern blot hybridization and by the loss of plasmid methylation. In addition, transformed strains retained their capacity of infecting Serrano pepper seedlings and Mc. Intosh apple fruits, the host plants for P.capsici and P.parasitica, respectively.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=328573Documentos Relacionados
- Arbuscular mycorrhizal fungi communities in citrus conventional and organic farming and their interactions with Phytophthora parasitica.
- Seleção de plantas resistentes e de fungicidas para o controle da "morte prematura" do maracujazeiro, causada por Nectria haematococca e Phytophthora parasitica.
- Defence genes in Citrus sunki and Poncirus trifoliata: expression constitutive and induced by Phytophthora parasitica.
- Nucleotide sequence of the glyceraldehyde-3-phosphate dehydrogenase gene from Cryphonectria parasitica.
- Terminal structure of hypovirulence-associated dsRNAs in the chestnut blight fungus Endothia parasitica.
