Genetic selection for genes encoding sequence-specific DNA-binding proteins.
AUTOR(ES)
Elledge, S J
RESUMO
We describe a genetic selection method designed to facilitate the cloning of genes encoding sequence-specific DNA-binding proteins. The strategy selects for clones expressing particular sequence-specific DNA-binding activities from a library of clones encoding other, nonspecific proteins. Specific DNA-binding sites have been placed near the start of transcription of the strong synthetic conII promoter to create promoters that can be repressed by the corresponding sequence-specific DNA-binding proteins. Transcription from the conII derivatives in the absence of repression interferes with the phenotypic expression of an adjacent drug-resistance gene, aadA. Sequence-specific DNA-binding proteins are shown to repress these promoters and alleviate transcriptional interference of aadA, resulting in drug resistance in cells expressing the appropriate DNA-binding protein.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=287205Documentos Relacionados
- Novel method for identifying sequence-specific DNA-binding proteins.
- A high-capacity column for affinity purification of sequence-specific DNA-binding proteins.
- Sequence-specific DNA-binding dominated by dehydration.
- Potent stimulation of transcription-coupled DNA supercoiling by sequence-specific DNA-binding proteins
- Sequence-specific DNA binding by Myc proteins.