Genetic Relatedness of Type 1 and Type 2 Herpes Simplex Viruses

AUTOR(ES)

Kieff, Elliott

RESUMO

The extent of homology between herpes simplex virus1 and2 (HSV-1 and HSV-2) deoxyribonucleic acid (DNA) was measured in two ways: (i) by determination of the relative rate of hybridization of labeled HSV-1 and HSV-2 DNA to excess unlabeled HSV-1 or HSV-2 DNA immobilized on filters and (ii) by determination of the rate of hybridization of labeled HSV-1 and HSV-2 DNA to excess unlabeled HSV-1 or HSV-2 DNA in solution. Approximately 40% of HSV-1 and HSV-2 DNA is homologous at hybridization temperatures 25 C below the melting temperature (Tm) of HSV DNA (liquid-filter annealing). Lowering the temperature to 34 C below the Tm increased the extent of homology to 46% (liquid annealing). The extent of base-pairing in HSV-1-HSV-2 heteroduplex DNA was determined by thermal chromatography on hydroxyapatite. Heteroduplexes of HSV-1 and HSV-2 DNA eluted in a single peak whose midpoint (Te50) was 10 C below that of the homoduplex. Conspicuously absent were heteroduplexes that eluted at more than 15 C below the Te50 of the homoduplex. The data indicate the existence of a variable region of DNA (54%) with very little, if any, homology and an invariable region (46%) with relatively good (85%) matching of base pairs.

Documentos Relacionados

• Type 1 and Type 2 Herpes Simplex Viruses: Serological and Biological Differences 1
• Different susceptibilities of skin to type 1 and type 2 herpes simplex viruses in newborn rabbits.
• Phylogenetic Analysis of Clinical Herpes Simplex Virus Type 1 Isolates Identified Three Genetic Groups and Recombinant Viruses
• Differences in attachment between herpes simplex type 1 and type 2 viruses to neurons and glial cells.
• Nonvirion Antigens Produced by Herpes Simplex Viruses 1 and 2