Genetic mapping of the chromosomal replication origin of Salmonella typhimurium.

AUTOR(ES)

Joh, K

RESUMO

Two hundred strains of Escherichia coli harboring Filv+ plasmids which carry a segment of the Salmonella typhimurium chromosome were isolated independently. Among them, two strains were found to harbor F' plasmids that are able to replicate in Hfr cells of E. coli; i.e., they carry a site designated poh (permissive on Hfr) of the S. typhimurium chromosome. The poh site is presumably identical with the replication origin (oriC) of the bacterial chromosome. These two plasmids carry the dnaA-uncA-rbs-ilv-cya-metE region of the chromosome of S. typhimurium. Other F' plasmids which only carried the ilv-cya-metE region were unable to be maintained in Hfr cells. The poh site (= oriC) of S. typhimurium thus is located in the uhp-ilv region of the chromosome. The two plasmids carrying the poh site of S. typhimurium can suppress the temperature-sensitive character of an E. coli mutant that carries the temperature-sensitive dnaA46 allele, when the plasmids exist in the mutant cells. This suggests that the dnaA chromosome in place of the dnaA gene product of E. coli itself. The ability of the plasmids carrying the poh site of S. typhimurium to replicate in Hfr cells of E. coli suggests that the replication system of E. coli can recognize the Salmonella replication origin.

Documentos Relacionados

• Isolation and mapping of plasmids containing the Salmonella typhimurium origin of DNA replication.
• Mapping of the hemE locus in Salmonella typhimurium.
• The genetic control of DNA supercoiling in Salmonella typhimurium.
• Intracellular replication is essential for the virulence of Salmonella typhimurium.
• Primary structure and mapping of the hupA gene of Salmonella typhimurium.