Genetic diversity and reaction of Passiflora Spp. to M. Incognita and M. Javanica / Diversidade Genética e reação de Passiflora Spp. a Meloidogyne Incognita e a Meloidogyne Javanica




Considering that growing passion fruit has faced problems due to soil pathogens, responsible for significative losses, one objective of this study was to evaluate the reaction of four progenies of sour passion fruit (Passiflora edulis f. flavicarpa), one of sweet passion fruit (Passiflora alata), nine wild species of Passiflora (P. amethystina, P. setacea, P. nitida, P. serratodigitata, P. caerulea, P. gibertii, P. odontophylla, P.coccinea and a native genotype of P. edulis edulis ) and one interspecific hybrid (P. coccinea X P. stacea) in relation to Meloidogyne incognita and to Meloidogyne javanica). Two experiments were conducted under an experimental randomized design with split-plot arrangement. Seedlings with 90 days obtained from seeds or cuttings were inoculated and evaluated 90 days after inoculation, taking in account vegetative parameters of plant development, root galling and nematode reproduction. In general, there was significant influence on vegetative development of the different genotypes, mainly due to M. incognita, that showed the highest multiplication ratios and contributed to poor growth of inoculated pants. Taking the reproduction factor as a measure to classify levels of plant resistance P. amethystina, P. coccinea, P. serratodigitata, P. nitida, P. caerulea and P. gibertii were susceptible to M.incognita and all the species studied were resistant to M. javanica. Another objective of this work was to evaluate the genetic diversity of Passiflora spp., both based on the use of molecular markers RAPD (Random Amplified Polymorphic DNA) and RGAs (resistance genes analogs). Two experiments were planned by using PCR amplification. For the first one (RAPD), eight primers were used to obtain 194 amplicons, all of them were polymorphic. In the second experiment, six primer combinations with RGAs as molecular markers, resulted in the formation of 99 amplicons. Nine-six of them were polymorphic, while three were monomorphic. Therefore, sources of RAPD and RGAs molecular markers were detected among Passiflora genotypes, with potential application in breeding programs towards resistance to plant diseases.


meloidogyne javanica meloidogyne javanica meloidogyne incognita ciências biológicas resistance marcadores moleculares meloidogyne incognita passiflora molecular markers resistência passiflora

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