Genetic characterization of St. Louis encephalitis virus isolated from human in São Paulo, Brazil
AUTOR(ES)
Santos, Cecília Luiza Simões dos, Sallum, Maria Anice Mureb, Franco, Heitor Moreira, Oshiro, Fabíola Maiumi, Rocco, Iray Maria
FONTE
Memórias do Instituto Oswaldo Cruz
DATA DE PUBLICAÇÃO
2006-02
RESUMO
The molecular characterization of SPH253157, a new strain of St. Louis encephalitis virus (SLEV), isolated in 2004 from the first case of human infection recognized in the state of São Paulo, Brazil, is reported. The patient, presenting a febrile illness without neurological involvement, was hospitalized as a probable case of dengue fever. Genomic RNA was isolated from the supernatant of C6/36 cells infected with acute phase-serum specimen of the patient and the envelope gene was amplified by reverse-transcription-polymerase chain reaction. The complete nucleotide sequence of the envelope gene of this isolate was directly sequenced from the amplified products and compared with other Brazilian and American SLEV strains. Phylogenetic analyses were carried out under maximum likelihood criterion with outgroups both included and excluded. Outgroups comprised four flavivirus of the Japanese encephalitis group. Phylogeny also included Bayesian analysis. The results indicated that the new SLEV isolate belongs to lineage III, being closely related to an Argentinean strain recovered from Culex sp. in 1979. It is concluded that there are at least 3 lineages of SLEV in Brazil.
Documentos Relacionados
- Stability of St. Louis Encephalitis Virus in the Airborne State
- Experimental Transplacental Transmission of St. Louis Encephalitis Virus in Mice 1
- Use of Base Excision Sequence Scanning for Detection of Genetic Variations in St. Louis Encephalitis Virus Isolates
- Opsoclonus in a confirmed case of St. Louis encephalitis.
- Serological detection of St. Louis encephalitis virus and West Nile virus in equines from Santa Fe, Argentina