Genetic and fisiological characterization de Crinipellis perniciosa. / Caracterização genética e fisiológica de Crinipellis perniciosa.

AUTOR(ES)

Taís Guimarães Lana

DATA DE PUBLICAÇÃO

2004

RESUMO

The basidiomycete fungus Crinipellis perniciosa (Stahel) Singer is the causal agent of Witches Broom Disease of Cacao (Theobroma cacao L.) which is the main factor limiting cocoa production in the Americas. Pod losses of up to 90% are experienced in affected areas as evidenced by the 50% drop in production in Bahia province, Brazil following the arrival of the C. perniciosa in the area in 1989. The disease has proven particularly difficult to control and many farmers in affected areas have given up cacao cultivation. Any useful control strategy for Witches Broom disease must be effective against in range strains of the pathogen. It is already known that pathogenic variation exists among isolates of Crinipellis perniciosa obtained from different areas and host plants. However, any study was developed about the variability between endophytic and pathogenic population. In order to evaluate the genetic variability of 35 isolates of endophytic and pathogenic populations of Crinipellis perniciosa, the RAPD technique, ITS sequencing and fungicide susceptibility were performed. Genetic variability between 35 isolates of C. perniciosa was analysed by the random amplified polymorphic DNA (RAPD) technique, which indicated that isolates from other host plants were more diverse than isolates obtained from cacao plants. Among cacao isolates was observed at least two groups, one formed mainly by endophytic isolates and other by pathogenic ones. Analysis by ITS sequence grouped isolates independently of endophytic or pathogenic status. Fungicide susceptibility showed that cupric oxide inhibits statistically more endophytic isolates than pathogenic ones, showing that could have physiological differences between these populations. The present study highlighted the possible genetic and physiological differences between endophytic and pathogenic population of C. perniciosa.

ASSUNTO(S)

phytopathogenic fungi cacao dna genética microbiana enzyme witches broom fungicide genetic variation microbial genetic agromicrobiology dna fungicida cacau fungo fitopatogênico enzima microbiologia agrícola variação genética vassoura-de-bruxa

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