Genetic analysis of the human cell surface: antigenic marker for the human X chromosome in human-mouse hybrids.
AUTOR(ES)
Dorman, B P
RESUMO
Somatic cell hybrids of human fibroblasts and mouse A9 cells, carrying only a portion of the human X chromosome in a mouse chromosome background, were injected into C3H mice. The resulting mouse anti-hybrid cell antisera contain antibodies found to be human specific and to react with only those hybrid cells carrying the human X chromosome, as confirmed by essentially perfect concordance between antibody binding assayed by indirect immunofluorescence and presence of the human X-linked enzyme hypoxanthine phosphoribosyl-transferase determined by autoradiographic assay of [3H] hypoxanthine utilization. Heterogeneous mixtures of hybrid cells may be analyzed into fluorescent (X plus) and nonfluorescent (X minus) subpopulations and fractionated viably by using a fluorescence-activated cell sorter.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=392553Documentos Relacionados
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