Genes regulating the plant cell cycle: isolation of a mitotic-like cyclin from Arabidopsis thaliana.
AUTOR(ES)
Hemerly, A
RESUMO
A key element of cell cycle control in eukaryotes is the M-phase kinase, composed of p34cdc2 and cyclin. To dissect the plant cell cycle, we have previously isolated a cdc2 gene homolog from Arabidopsis thaliana. We have now cloned an Arabidopsis cDNA corresponding to cyclins. This gene (cyc1At) encodes a protein with a predicted molecular mass of 48.4 kDa and a domain homologous to the cyclin box of mitotic cyclins. However, by sequence comparison the cyc1At gene could not be assigned to the A- or B-type group. The mRNA accumulates preferentially in actively dividing cells and when these cells are blocked during the cell cycle, the amount of transcripts decreases dramatically. cyc1At mRNA is found mainly in G2-phase nuclei, suggesting that its expression is periodic in the cell cycle. Microinjection of synthetic cyc1At mRNA induced meiotic maturation in Xenopus oocytes. Cyc1At is encoded by a single gene, but the amplification by the polymerase chain reaction of other fragments homologous to cyclins indicates the presence of a family of cyclins in Arabidopsis.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=48853Documentos Relacionados
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