Genes encoding the phycobilisome rod substructure are clustered on the Anabaena chromosome: characterization of the phycoerythrocyanin operon.

AUTOR(ES)
RESUMO

The phycoerythrocyanin (pec) operon, cloned from Anabaena sp. strain PCC 7120, encodes four genes, pecBACE, located upstream of the C-phycocyanin (cpc) operon. This pec-cpc cluster includes all the genes for the structural components of the phycobilisome rod. Oligonucleotide probes based on the amino-terminal sequence of the phycoerythrocyanin beta subunit were used to clone an 8.0-kbp EcoRI fragment which was determined, by sequencing, to partially overlap the previously cloned cpc operon. A 5.0-kbp EcoRI-ClaI fragment corresponding to the region upstream of the cpc operon was subsequently subcloned and sequenced. Five open reading frames whose polarity of transcription is parallel to that of the cpc genes were identified. pecB and pecA encode the beta and alpha subunits of phycoerythrocyanin, respectively. pecC encodes the phycoerythrocyanin-associated linker polypeptide LR34.5,PEC. The identities of these genes are confirmed by agreement with amino-terminal sequences determined from purified phycobilisome components. A gene homologous to cpcE, found downstream of pecC, has been designated pecE. The cpcE gene product is involved in the attachment of the phycocyanobilin chromophore to the alpha subunit of phycocyanin. Three transcripts were observed by Northern (RNA) analyses. The most abundant of these transcripts, 1.35 kbp, corresponds to the beta and alpha subunit genes, whereas the less-abundant transcripts, 2.3 and 3.1 kbp, correspond to pecBAC and pecBACE, respectively. Phycoerythrocyanin is strongly induced in cells cultured under low light. In parallel, all three transcripts were present at much higher levels in cells cultured under low light.

ACESSO AO ARTIGO

Documentos Relacionados