Genes ABI3(ABL-Interactor Member 3)e ABI3BP (ABI3-Binding Protein): investigação da expressão em lesões da tiróide e análise funcional. / Genes ABI3 (GLA Member Interactor-3) and ABI3BP (ABI3-Binding Protein): investigation of expression in thyroid lesions and functional analysis.

AUTOR(ES)
DATA DE PUBLICAÇÃO

2009

RESUMO

We have recently demonstrated that loss of expression of the ABI3BP (ABI3-binding protein) gene is common to all thyroid carcinomas, and that ABI3BP re-expression altered tumor growth by increasing senescence and inhibiting invasion and migration. ABI3 is a potential binding partner of ABI3BP and it has been shown that ABI3 forced expression reduces motility and metastatic dissemination of malignant cells. In this study we investigated ABI3 expression in thyroid lesions. We observed that ABI3 expression is reduced or lost in most carcinomas and that there is a positive correlation between ABI3 and ABI3BP expression. The role of ABI3 was further explored by stable transfection of two carcinoma cell lines with full-length ABI3 cDNA. We found that ABI3 decreases transforming activity, cell proliferation and cell cycle progression while increasing cell senescence correlated with increased p21 expression and reduced ERK phosphorylation and E2F1 expression. Moreover, ectopic expression reduces cell viability, cell invasion and migration. In rodent models, ABI3 expression was sufficient to reduce tumor growth. Here, we suggest that ABI3 loss of expression, similar to ABI3BP loss of expression, is an important event in thyroid carcinoma. This work establishes a functional role for ABI3 in thyroid cancers and suggests that targeting ABI3 or its pathway might be a strategy for the treatment of these cancers.

ASSUNTO(S)

abi3bp, abi3, tarsh, nesh - câncer de tiróide neoplasias foliculares linhagens celulares da tiróide análise funcional morfologia

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