Generation of a triple-fluorescent mouse strain allows a dynamic and spatial visualization of different liver phagocytes in vivo
AUTOR(ES)
NAKAGAKI, BRENDA N., FREITAS-LOPES, MARIA A., CARVALHO, ÉRIKA, CARVALHO-GONTIJO, RAQUEL, CASTRO-OLIVEIRA, HORTÊNCIA M., REZENDE, RAFAEL M., CARA, DENISE C., SANTOS, MÔNICA M., LOPES, RODRIGO PESTANA, DAVID, BRUNA A., MENEZES, GUSTAVO B.
FONTE
An. Acad. Bras. Ciênc.
DATA DE PUBLICAÇÃO
16/10/2017
RESUMO
ABSTRACT Resident and circulating immune cells have been extensively studied due to their almost ubiquitous role in cell biology. Despite their classification under the “immune cell department”, it is becoming increasingly clear that these cells are involved in many different non-immune related phenomena, including fetus development, vascular formation, memory, social behavior and many other phenotypes. There is a huge potential in combining high-throughput assays - including flow cytometry and gene analysis - with in vivo imaging. This can improve our knowledge in both basic and clinical cell biology, and accessing the expression of markers that are relevant in the context of both homeostasis and disease conditions might be instrumental. Here we describe how we generated a novel mouse strain that spontaneously express three different fluorescence markers under control of well-studied receptors (CX3CR1, CCR2 and CD11c) that are involved in a plethora of stages of cell ontogenesis, maturation, migration and behavior. Also, we assess the percentage of the expression and co-expression of each marker under homeostasis conditions, and how these cells behave when a local inflammation is induced in the liver applying a cutting-edge technology to image cells by confocal intravital microscopy.
Documentos Relacionados
- Effect of mouse phagocytes on Candida albicans in in vivo chambers.
- Inhibition of NF-κB Activation in Combination with Bcl-2 Expression Allows for Persistence of First-Generation Adenovirus Vectors in the Mouse Liver
- Dynamic in vivo imaging and cell tracking using a histone fluorescent protein fusion in mice
- Insertion of Green Fluorescent Protein into Nonstructural Protein 5A Allows Direct Visualization of Functional Hepatitis C Virus Replication Complexes†
- Adenovirus-mediated urokinase gene transfer induces liver regeneration and allows for efficient retrovirus transduction of hepatocytes in vivo.