Generation of a Recombinant Cytomegalovirus for Expression of a Hantavirus Glycoprotein
AUTOR(ES)
Rizvanov, Albert A.
FONTE
American Society for Microbiology
RESUMO
A cytomegalovirus (CMV) was isolated from its natural host, Peromyscus maniculatus, and was designated Peromyscus CMV (PCMV). A recombinant PCMV was constructed that contained Sin Nombre virus glycoprotein G1 (SNV-G1) fused in frame to the enhanced green fluorescent protein (EGFP) gene inserted into a site homologous to the human CMV UL33 (P33) gene. The recombinant CMV was used for expression and immunization of deer mice against SNV-G1. The results of the study indicate that P. maniculatus could be infected with as few as 10 virus particles of recombinant virus. Challenge of P. maniculatus with either recombinant or wild-type PCMV produced no overt pathology in infected animals. P. maniculatus immunized with recombinant virus developed an antibody response to SNV and EGFP. When rechallenged with recombinant virus, animals exhibited an anamnestic response against SNV. Interestingly, a preexisting immune response against PCMV did not prevent reinfection with recombinant PCMV.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=254267Documentos Relacionados
- Transfection-mediated generation of functionally competent Tula hantavirus with recombinant S RNA segment
- Identification of the murine cytomegalovirus glycoprotein B gene and its expression by recombinant vaccinia virus.
- Identification and expression of a human cytomegalovirus early glycoprotein.
- Expression and characterization of recombinant murine cytomegalovirus protease.
- Evaluation of a Cytomegalovirus Glycoprotein B Recombinant Enzyme Immunoassay To Discriminate between a Recent and a Past Infection