General Method for the Identification of Plasmid Species in Fast-Growing Soil Microorganisms

AUTOR(ES)

Plazinski, Jacek

RESUMO

Using a horizontal gel electrophoresis method, we demonstrated reproducibly the presence of indigenous plasmids in different Rhizobium, Agrobacterium, and Pseudomonas strains. The method yields a large amount of plasmid DNA and is sensitive in detecting megaplasmids with molecular weights higher than 5 × 108. In two Rhizobium meliloti strains, a megaplasmid other than the low-mobility plasmid already known was detected.

Documentos Relacionados

• Underexpression of Ap from R-Plasmids in Fast-Growing Rhizobium Species
• Beta-2-linked glucans secreted by fast-growing species of Rhizobium.
• Mobilization of a Sym plasmid from a fast-growing cowpea Rhizobium strain.
• Efficient homologous recombination in fast-growing and slow-growing mycobacteria.
• Growth-Related Substituent Changes in Exopolysaccharides of Fast-Growing Rhizobia