Gene targeting restricted to mouse striated muscle lineage.
AUTOR(ES)
Miniou, P
RESUMO
Spatially and temporally regulated somatic mutations can be achieved by using the Cre/LoxP recombination system of bacteriophage P1. In order to develop gene knockouts restricted to striated muscle, we generated a transgenic mouse line expressing Cre recombinase under the control of the human alpha-skeletal actin promoter. Specific excision of a loxP-flanked gene was demonstrated in striated muscle, heart and skeletal muscle, in a pattern very similar to the expression of the endogenous alpha-skeletal actin gene. Therefore, the reported transgenic line can be used to target inactivation or activation of a given gene to the skeletal muscle lineage.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=148637Documentos Relacionados
- Sialoadhesin binds preferentially to cells of the granulocytic lineage.
- Thy-1+ dendritic epidermal cells belong to the T-cell lineage.
- T cell receptor (TCR) gene rearrangements in the assignment of tumour cell lineage.
- v-jun cooperates with v-erbB to transform the thrombocytic/megakaryocytic lineage.
- Multiple occurrences of mutualism in the yucca moth lineage.