Gating kinetics of the quisqualate-sensitive glutamate receptor of locust muscle studied using agonist concentration jumps and computer simulations.

AUTOR(ES)

Standley, C

RESUMO

Outside-out patches excised from extrajunctional membrane of locust muscle were subjected to "concentration jumps" of L-glutamate, using the liquid filament switch technique, to study channel opening and closing rates, desensitization onset, and recovery from desensitization of a quisqualate-sensitive glutamate receptor (qGluR). Based on data obtained from these experimental studies, computer modeling techniques have been used in an attempt to simulate the behavior of qGluR during a concentration jump of L-glutamate. A linear model with three closed states (one unliganded, one monoliganded, and one biliganded), one open state (binding two molecules of L-glutamate), and two desensitization states (the one monoliganded, the other biliganded) leading from the unliganded closed state simulated all of the experimentally observed behavior. The results are discussed in the context of previous equilibrium studies in which desensitization was inhibited with concanavalin A and for which a ten-state model was required to simulate the behavior of qGluR.

Documentos Relacionados

• Evolutionary games and computer simulations.
• Role of water on unfolding kinetics of helical peptides studied by molecular dynamics simulations.
• Dependence of acetylcholine receptor channel kinetics on agonist concentration in cultured mouse muscle fibres.
• Structural determinants of agonist-specific kinetics at the ionotropic glutamate receptor 2
• Hydration forces between parallel DNA double helices: computer simulations.