Ganciclovir-resistant cytomegalovirus clinical isolates: mode of resistance to ganciclovir.
AUTOR(ES)
Stanat, S C
RESUMO
Cytomegalovirus strains with reduced in vitro susceptibilities to ganciclovir have been recovered from patients who failed long-term ganciclovir therapy. The ganciclovir-resistant clinical isolates in this study were unable to induce ganciclovir phosphorylation in virus-infected cells. The viral DNA polymerase function appeared unaltered in one genetically pure ganciclovir-resistant strain, compared with that of its wild-type ganciclovir-sensitive counterpart. All nine of the ganciclovir-resistant strains were susceptible to foscarnet. Moreover, these strains were sensitive to inhibition both by vidarabine and 1-(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)-5-iodocytosine (FIAC), antiviral agents that are activated by cellular enzymes, and by (S)-1(3-hydroxy-2-phosphonylmethoxypropyl)cytosine (HPMPC), which is a monophosphate nucleoside analog. The in vitro resistance to ganciclovir of the ganciclovir-resistant clinical isolates studied was attributed to the inability of the cells infected with these isolates to phosphorylate ganciclovir; the virally encoded DNA polymerase did not appear to play a role in this ganciclovir resistance.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=245358Documentos Relacionados
- High Impact of an Expanded Restriction Fragment Length Polymorphism Assay on Detection of Ganciclovir-Resistant UL97 Mutants of Human Cytomegalovirus
- Novel mutation in the UL97 gene of a clinical cytomegalovirus strain conferring resistance to ganciclovir.
- Emergence of Multiple Human Cytomegalovirus Ganciclovir-Resistant Mutants with Deletions and Substitutions within the UL97 Gene in a Patient with Severe Combined Immunodeficiency
- Mutation in the UL97 open reading frame of human cytomegalovirus strains resistant to ganciclovir.
- Home treatment of cytomegalovirus retinitis with intravenous Ganciclovir.