Gamma-T4 hybrid bacteriophage carrying the thymidine kinase gene of bacteriophage T4.
AUTOR(ES)
Mileham, A J
RESUMO
Among 32 lambda-T4 recombinant phages selected for growth on a thymidylate synthetase-deficient (thyA) host, 2 were shown to carry the T4 thymidine kinase (tk) gene. The lambda-T4tk phages contain two T4 HindIII DNA fragments (2.0 and 1.5 kilobases) that hybridize to restriction fragments of T4 DNA, encompassing the tk locus at 60 kilobases on the T4 map. The T4tk insert compensates for the simultaneous host deficiencies of thymidine kinase and thymidylate synthetase in a thymidine kinase-deficient (tdk) host growing in the presence of fluorodeoxyuridine when provided with thymidine and uridine. The lambda-T4tk hybrid phages specified five polypeptides with Mrs of 22,000 (22K), 21K, 14K, 11K, and 9K.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=255686Documentos Relacionados
- Nucleotide sequence of the alt gene of bacteriophage T4.
- The immunity (imm) gene of Escherichia coli bacteriophage T4.
- The genome of bacteriophage T4.
- Isolation of mutants of bacteriophage T4 unable to induce thymidine kinase activity. II. Location of the structural gene for thymidine kinase.
- Intervening sequence in the thymidylate synthase gene of bacteriophage T4.