Galactose-containing polysaccharides from the human pathogens Sporothrix schenckii and Ceratocystis stenoceras.
AUTOR(ES)
Mendonça-Previato, L
RESUMO
Galactose-containing polysaccharides from three strains of Sporothrix schenckii and one strain of Ceratocystis stenoceras were isolated, and their structures were paritally characterized by chemical analysis and 13C-nuclear magnetic resonance spectroscopy (13C-NMR). S. schenckii polysaccharide preparations from all strains were not precipitated by Fehling solution and contained galactomannan (or a mixture of galactan and galactomannan), amylose, and minor amounts of rhamnomannan. C. stenoceras polysaccharide contained galactomannan and a smaller proportion of amylose. Conventional chemical techniques and 13C-NMR spectroscopy showed that the structures of the two preparations were closely related. The core of the galactomannan consisted principally of nonreducing end units and 2-O-, 2,6-di-O-, and perhaps 2,3-di-O-substituted alpha-D-mannopyranosyl units. The core was substituted by beta-D-galactofuranosyl chains; the units are interlinked (1 leads to 6). 13C-NMR evidence shows that the alpha-D-mannopyranosyl units are substituted in the two positions by the beta-D-galactofuranosyl residues. Galactomannans present at the cell surface of S. schenckii represent other potential fungal antigens in addition to the already recognized rhamnomannans and their peptide complexes.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=551220Documentos Relacionados
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