Functional Genes of Microorganisms, Comprehending the Dynamics of Agricultural Ecosystems

AUTOR(ES)

Reali, Catiusca, Pilz Júnior, Harry Luiz, Meile, Jean Christophe, Galindo, Sabine Shorr, Fiuza, Lidia Mariana

FONTE

Braz. arch. biol. technol.

DATA DE PUBLICAÇÃO

02/05/2017

RESUMO

ABSTRACT The microbial composition of different types,in ecosystems (including agro-ecosystems), has been investigated in a rapidly growing number of studies in the past few years. The importance of microorganisms, regarding the maintenance and stability of nutrients in agroecosystems, is a key to maintain the sustainability of a crop. Molecular tools to study microbial communities are possible through many methods such as RISA, DGGE, TGGE, clone libraries, T-RFLP, RAPD, SSCP and more recently NGS (Next-Generation Sequencing). DGGE is widely employed to characterize the diversity and the community dynamics of microorganisms in the environment, making possible to find out specific groups through functional genes, allowing access to data that cannot be obtained by cultural methods. The aim of this paper is to review the functional groups related to agroecosystems and to indicate the critical choice of DNA primers pairs and targeted DNA regions that may be used in PCR-based methods such as the DGGE technique in order to evaluate the microbial communities in a variety of environments.

Documentos Relacionados

• The University of Minnesota Biocatalysis/Biodegradation Database: microorganisms, genomics and prediction
• Transfer of Microorganisms, Including Listeria monocytogenes, from Various Materials to Beef
• ENZYMES FOR FORMATION OF CITROVORUM FACTOR IN MEMBERS OF THE PSITTACOSIS GROUP OF MICROORGANISMS, 12
• Microbiological Determination of Vitamins and Amino Acids Produced by Microorganisms, Using the Dialysis Cell1
• Application of microorganisms, alone or in combination, to control postbloom fruit drop in citrus