Functional expression of the cre-lox site-specific recombination system in the yeast Saccharomyces cerevisiae.
AUTOR(ES)
Sauer, B
RESUMO
The procaryotic cre-lox site-specific recombination system of coliphage P1 was shown to function in an efficient manner in a eucaryote, the yeast Saccharomyces cerevisiae. The cre gene, which codes for a site-specific recombinase, was placed under control of the yeast GALI promoter. lox sites flanking the LEU2 gene were integrated into two different chromosomes in both orientations. Excisive recombination at the lox sites (as measured by loss of the LEU2 gene) was promoted efficiently and accurately by the Cre protein and was dependent upon induction by galactose. These results demonstrate that a procaryotic recombinase can enter a eucaryotic nucleus and, moreover, that the ability of the Cre recombinase to perform precise recombination events on the chromosomes of S. cerevisiae is unimpaired by chromatin structure.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=365329Documentos Relacionados
- Site-specific cleavage of chromosomes in vitro through Cre-lox recombination.
- Intra-chromosomal rearrangements generated by Cre-lox site-specific recombination.
- Seed-specific gene activation mediated by the Cre/lox site-specific recombination system.
- Using the cre-lox recombination system to assess functional impairment caused by amino acid substitutions in yeast proteins
- A new approach for the identification and cloning of genes: the pBACwich system using Cre/lox site-specific recombination