Functional Expression of hMYH, a Human Homolog of the Escherichia coli MutY Protein

AUTOR(ES)

Slupska, Malgorzata M.

FONTE

American Society for Microbiology

RESUMO

We have previously described the hMYH cDNA and genomic clones (M. M. Slupska et al., J. Bacteriol. 178:3885–3892, 1996). Here, we report that the enzyme expressed from an hMYH cDNA clone in Escherichia coli complements the mutator phenotype in a mutY mutant and can remove A from an A · 8-hydroxydeoxyguanine mismatch and to a lesser extent can remove A from an A · G mismatch in vitro.

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