Functional Analysis of Tomato Pti4 in Arabidopsis12
AUTOR(ES)
Wu, Keqiang
FONTE
American Society of Plant Physiologists
RESUMO
Pti4 is a tomato (Lycopersicon esculentum) transcription factor that belongs to the ERF (ethylene-responsive element binding factor) family of proteins. It interacts with the Pto kinase in tomato, which confers resistance to the Pseudomonas syringae pv tomato pathogen that causes bacterial speck disease. To study the function of Pti4, transgenic Arabidopsis plants were generated that expressed tomato Pti4 driven by the strong constitutive promoters, cauliflower mosaic virus 35S and tCUP. Global gene expression analysis by Affimetric GeneChip indicated that expression of Pti4 in transgenic Arabidopsis plants induced the expression of GCC box-containing PR genes. We also demonstrated that Pti4 enhanced GCC box-mediated transcription of a reporter gene. The data suggests that tomato Pti4 could act as a transcriptional activator to regulate expression of GCC box-containing genes. Furthermore, we show that the expression of tomato Pti4 in transgenic Arabidopsis plants produced a phenotype similar to that seen in plants treated with ethylene, thus providing evidence that the Pti4 gene is involved in the regulation of a subset of ethylene-responsive genes containing the GCC box.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=148941Documentos Relacionados
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