Frequent Human Genomic DNA Transduction Driven by LINE-1 Retrotransposition

AUTOR(ES)

Pickeral, Oxana K.

FONTE

Cold Spring Harbor Laboratory Press

RESUMO

Human L1 retrotransposons can produce DNA transduction events in which unique DNA segments downstream of L1 elements are mobilized as part of aberrant retrotransposition events. That L1s are capable of carrying out such a reaction in tissue culture cells was elegantly demonstrated. Using bioinformatic approaches to analyze the structures of L1 element target site duplications and flanking sequence features, we provide evidence suggesting that ∼15% of full-length L1 elements bear evidence of flanking DNA segment transduction. Extrapolating these findings to the 600,000 copies of L1 in the genome, we predict that the amount of DNA transduced by L1 represents ∼1% of the genome, a fraction comparable with that occupied by exons.

Documentos Relacionados

• Reading between the LINEs: Human Genomic Variation Induced by LINE-1 Retrotransposition
• Retrotransposition-Competent Human LINE-1 Induces Apoptosis in Cancer Cells With Intact p53
• Genomic Characterization of Recent Human LINE-1 Insertions: Evidence Supporting Random Insertion
• Translation of LINE-1 DNA elements in vitro and in human cells.
• Methyl-CpG-binding protein 2 represses LINE-1 expression and retrotransposition but not Alu transcription